The E3 ubiquitin ligase NLA postively regulates cold tolerance and negatively regulates phosphate uptake in maize, and a genetically engineered variant of this enzyme leads to improved cold tolerance and enhanced phosphate uptake, improving yield in field trials.
Chained 3× transforms (8KB × 500)
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If the A* calculation for a shortcut (in Step 3) finds it's now impassable, or if its actual detailed cost is significantly different (e.g., 20%) from the pre-calculated shortcut value:
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